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Chemical Equivalence Of Different Brands Of Oxytertacycline Hydrochloride And Its Minimum

by Sadaf Hina | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Dr. Muhammad Adil Rasheed.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2012Dissertation note: This project was designed to study the chemical equivalence of various brands of Oxytetracycline hydrochloride (long acting, short acting & PVP) approved by the ministry of health and available in the local market for veterinary use. Oxytetracycline was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the Oxytetracycline by HPLC assay method were determined. From stock solution of working standard (Oxytetracycline hydrochloride) different concentrations 0.05, 0.1, 0.5, 1.0, 10, 25, 50 and 100µg per ml were prepared for the determination of LOD. The LOD calculated was 0.100(µg/ml) and LOQ was 0.5 (µg/ml). Correlation coefficient was 0.99994050. Concentration of the active ingredient (Oxytetracycline hydrochloride) in all preparations was same as mentioned on the label except Oxytetracycline (74%), Terrasym PVP-100 (81%), and Onyx-LA (72%). MIC of Oxytetracycline hydrochloride against following bacterial isolates determined by micro-broth dilution test was Bacillus subtilis (50µg), Staphylococcus aureus (100µg), Eschericiha coli (50µg), Salmonella enterica (1000µg) and Pasturella multocida (50µg).It showed that all these bacterial cultures have developed resistance against Oxytetracycline hydrochloride. Availability: Items available for loan: UVAS Library [Call number: 1468,T] (1).

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Reversal Of Antibiotics Resistance In Methicillin-Resistant Staphylococcus Aureus By Non-Antimicrobial Agents

by Sohaib Danyaal | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Dr. Muhammad Adil Rasheed.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Antibiotic resistance is increasing along with the increasing use of antibiotic for the treatment of infectious diseases. The in-vitro study was designed to observe the reversal of antibiotics resistance in Methicillin-resistant Staphylococcus aureus by non-antimicrobial agents. One hundred pus samples were processed for isolation and identification of MRSA. Out of 100, 37 (37%) islotes were Gram +ve cocci, from these 37 isolates 34 (92%) turn red colour of mannitol salt media to yellow and 23 (62%) gave +ve catalase and coagulase. In this study Antibiotic Sensitivity Test was performed on pure culture of MRSA strain by applying disc diffusion method. Out of 23 pure MRSA isolate, 100% isolates were methacillin resistant, 79% isolates were co-amoxiclav resistant, 30% isolates were meropenum resistant, 8% isolates were vancomycin resistant, 26% isoltes were moxifloxacin resistant and 39% isolates were linezolid resistant. Reversal of antibiotics resistance was observed by MIC or serial dilution method, using non antibiotic agents like Amiloride, Lansoprazole and Promethazine, Concentrations of non-antibiotic agents 1024ìg, 512ìg, 256ìg, 128ug, 64ìg and 32ìg were used in combination of antibiotics to reverse the antibiotics resistance in MRSA. These non antibiotic agents may cause the alteration in mechanisms by which microorganism develop resistance. The collected data analyzed by applying analysis of variance (ANOVA) through SPSS 16.0 computer software. Now we would be able to treat some lethal infection caused by MRSA, and help to increase patient compliance and decrease the cost of therapy. Availability: Items available for loan: UVAS Library [Call number: 1828,T] (1).

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Role Of Non-Antimicrobial Agents In Reversal Of Antibiotic Resistance In Escherichia Coli

by Kalim Ullah | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Prof. Dr. Aftab.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1839,T] (1).

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Evaluation Of Antibacterial Activity Of Macrolides In Combination With Levamisole Hc1 Against Different Pathogenic Bacteria

by Sheeza javaid | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil rasheed | Proff. Dr. Aftab.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2026,T] (1).

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Synergistic Effect Of Antimicrobial Agents In Combination With Colistin Against Bacterial Isolates From Patients

by Sahar Safdar | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Prof. Dr. Aftab.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2123,T] (1).

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